Merge technical replicates (SRR1 + SRR2 -> SRX)

Open
#270 0 comments 0 reactions 0 assignees View on GitHub

Maintainers usually reply within 1 day

Nobody has claimed this yet.

Assessment

Difficulty
4/5
Estimated time
3-5 days
Newbie friendliness
45/100
Issue type
Feature
Clarity
Mostly clear
Activity status
Stale

Research direction

No source files or tests are named. Start by reading the linked fetchngs usage documentation and tracing how SRR/ERR/DRR identifiers resolve to SRX/ERX/DRX experiments. Done means technical replicate FASTQ files are concatenated per index within each experiment, including both R1 and R2 outputs.

Written by the indexing model from the issue text.

Description

enhancement
Description of feature

The feature I'm hoping for is merging of technical replicates / runs (SRRs) within each sample/experiment (SRX/GSM/etc). I would like the fastq files to be concatenated within each index (SRR1.R1.fq + SRR2.R1.fq -> SRX.R1.fq; SRR1.R2.fq + SRR2.R2.fq -> SRX.R2.fq). Is such a feature possible in the current pipeline? This would be really nice for those who wish to go directly from fetchngs to other nf-core pipelines.

The docs suggest this should be possible (link), but it doesn't seem to be implemented yet.

Dominant language
Nextflow
Stars
202
Forks
96
Avg merge
6h 36m
Merged PRs (30d)
4

Getting set up

Open in Codespaces

Starts the project's dev container in your browser, under your own GitHub account.

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

More from nf-core/fetchngs

All issues in nf-core/fetchngs

Similar issues

More Bioinformatics issues

Get new issues in your inbox

A short digest of beginner-friendly GitHub issues.