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MetaPathways is a meta'omic analysis pipeline for the annotation and analysis for environmental sequence information. MetaPathways is a modular annotation and analysis pipeline that uses a user-friendly graphical user interface and knowledge engine data structure to predict metabolic interaction networks from environmental sequence information. Currently, MetaPathways supports genomic read quality control, open reading frame (ORF) prediction, ORF annotation, and environmental pathway-genomes database (ePGDB) construction compatible with the Pathway Tools browser by SRI.