Unable to verify signature of AWS JWTs

Start with the TypeScript reproduction at jsonwebtoken.verify and compare its inputs and options with the Python jwt.decode call; the issue names no repository files or tests. Determine why the same ES256 JWT and PEM key produce different verification results, then document a reproducible cause and confirm matching verification behavior without weakening signature checks.

Description

I have some JWTs obtained from AWS/Cognito with the Elastic Load Balancer performing authentication for me. I also have the associated public key from the documented endpoints for the Cognito userpool. However the jsonwebtoken library says the signature is invalid (JsonWebTokenError: invalid signature), except when I use the same input (same JWT and public key in PEM format) in PyJWT Python code, this decodes/verifies without issue. There is no information to point to why jsonwebtoken is unable to verify the JWT/PEM when another library (PyJWT) can. I have tried ignoring any expiration checking in case this is the cause of the "invalid signature", such as ignoreExpiration, clockTolerance, and maxAge, without success. The only discrepancy I can see is the jsonwebtoken library must remove the base64 padding (=) from the JWT first.
Is there any known reason why jsonwebtoken would be unable to verify a signature which can be verified with another library? Perhaps some assumptions about the format/layout/syntax of the inputs which AWS/Cognito is not respecting.

Reproduction

Python code:

import jwt
import requests
import base64
import json

encoded_jwt = """eyJ0e
......
tE5Bg=="""

pub_key = """-----BEGIN PUBLIC KEY-----
MFkw
.....
J5og==
-----END PUBLIC KEY-----"""

payload = jwt.decode(encoded_jwt, pub_key, algorithms=['ES256'], leeway=10000)
print('Payload: '+ str(payload)) #This works fine

TypeScript code:

userJWT = userJWT.replace(/=/g, '');

// This fails, with  JsonWebTokenError: invalid signature
return jsonwebtoken.verify(userJWT, this.pem,
{algorithms: ['ES256'], ignoreExpiration: true, clockTolerance: 100000, maxAge: "1 day"});

Environment

Please provide the following:

• Version of this library used:
• jsonwebtokens=8.5.1
• PyJWT = 1.7.1
• Version of the platform or framework used, if applicable:
• Python 3.8.10
• Node v17.4.0
• Other relevant versions (language, server software, OS, browser):
• Ubuntu 21.04 (Kernel 5.14)
• Other modules/plugins/libraries that might be involved: